General

Biological Containment

1. Purpose: Containment facility for research on Biosafety Level 2 pathogens.
2. Location: University of Cincinnati College of Medicine
3. Director:  Neal Wolfe (558-3486) email: wolfen@ucmail.uc.edu
4. Fee Structure:  Hourly charge
5. Frequency of Use:  Unlimited
6. Accessibility:  Open to all qualified UC and select non-UC personnel

Biostatistical Services

1. Purpose: All statistical assistance towards grant preparation such as, formulating and phrasing hypotheses, devising appropriate study designs, defining outcome, predictor, and confounding variables,calculation of sample size and power, outlining data-analytic strategies and tests, and writing or editing sections of the grant proposal.
2. Location: Room G-27, Kettering Lab, ML #0056
3. Web site: www.med.uc.edu/biostatistics/
4. Director: Rakesh Shukla, Ph.D. (558-0108) email: rakesh.shukla@uc.edu
5. Consultant: Linda Levin, Ph.D. (558-0050) email: linda.levin@uc.edu
6. Fee structure: Fee-Structure: All assistance directly towards grant-proposal to be submitted for external funding, by the full-time faculty of the UC Academic Health Center is FREE. Other biostatistical services on non-proposal apsects such as consultations, advice, and assistance on data management and data analysis can be available and have hourly-rate.
7. Frequency of use: Undetermined at this time
8. Accessible to all full-time faculty at the University of Cincinnati Medical Center

Cell Manipulation Core
Digestive Disease Research Development Center (DDRDC)

1. Purpose: The DDRDC Cell Manipulation Core provides cells either from primary tissue harvest (liver or intestine) or existing established cell lines (intestinal, liver, and pancreatic) to members of the DDRDC.  This core specializes in modifying gene expression in cell culture. 
2. Location: CCHMC Building R (Research Foundation Building) Room 2030
3. Web site: http://www.cincinnatichildrens.org/research/project/ddrdc/cores/
4. Faculty Supervisor: Jorge A. Bezerra, MD (636-3008) email: jorge.bezerra@cchmc.org
5. Contact Person: Reena Mouray, MPharm (636-9731) email: reena.mourya@cchmc.org
6. Frequency of Use: Unlimited
7. Accessible to members of the Digestive Disease Research Development Center

Comparative Pathology

1. Purpose: Consult and carry out pathology studies on animals or animal tissues.
2. Location: Medical Science Building 1252 and 1005. (These rooms will change with the construction of the care building)
3. Faculty Supervisor: Dr. Greg Boivin, 558-9156; boivingp@ucmail.uc.edu Laboratory Supervisor: Angela Sklenka, 558-4436 Types of Preparations: Animal necropsy, tissue processing, embedding, sectioning, staining, immunohistochemistry, frozen-sectioning
4. Some studies available: Animal necropsy, tissue processing, embedding, sectioning, staining, immunohistochemistry, frozen-sectioning, photographs.
5. Fee Structure: Fee for service based on established schedule. Contact Dr. Boivin for more information. Technical support can also be added to grants directly
6. Frequency of Use: 1500 cases are received annually: 60% Medical Center, 25% Children’s Hospital, 10% VA Medical Center, 5% Shriners, West Campus and other Universities.
7. Web Page Link: http://pathology.uc.edu/divisions/comppath.asp
8. Accessible and available for sample submission during regular business hours.

Cytogenetics/Fish Laboratory

1. Primary purpose: Chromosome analyses/Gene mapping by FISH Clinical and Research Service
2. Location: CHMC/TCHRF 1012A (636-4474), ML# 0054, CHRF 1001
3. Faculty Supervisors: Dr. Howard Saal and Ruthann Blough, Ph.D., email: blour0@cchmc.org
4. Fee structure: Fee for service/collaborative arrangements
5. Frequency of use: Greater than 3,000 samples/year
6. Accessible: Sample referral arrangements through supervisors

Dietary Data Entry Center*

1. Purpose: Entry and analysis of dietary data including three-day diet records and dietary recalls.
2. Location: Children's Hospital Medical Center, The Research Foundation #7507, 3333 Burnet Avenue, Cincinnati, Ohio 45229
3. Faculty Supervisor: Dr. Stephen Daniels: E-Mail steve.daniels@cchmc.org Phone number 636-7272
4. Contact Person: Marcia Schmidt MS, RD, LD E-mail marcia.schmidt@cchmc.org Phone Number:636-4142
5. Fee Structure: $75 for a three day food record. Other services customized
6. Frequency of use: 80% outside, 20% inside work
7. Accessible to anyone needing services
8. Web Page Link: http://www.cincinnatichildrens.org/chrf/resources/ddec/

DNA Sequencing and Oligonucleotide Synthesis

1. Purpose: Synthesis and purification of oligonucleotides, nucleotide sequence analysis, contig assembly and sequencing template preparation, and mouse and human genotyping
2. Location: Department of Molecular Genetics, Biochemistry and Microbiology, Room 2302 MSB, ML# 0524
3. Faculty Supervisor: Dr. Joanna Groden (8-0088); email: joanna.groden@uc.edu, Director: Doug Bintzler (8-5520)
4. Fee structure: Fee for service established; supported by user charges
5. Frequency of use: Full-time, about 50% from outside the department of Molecular Genetics
6. Accessible to all funded investigators

General Clinical Research Center

1. Purpose: Provides facilities and resources for patient-oriented research Biostatistics core/Core Laboratory and Facilities for Bone Related Research
2. Location: CHMC, 3rd floor, ML# 0054
3. Faculty Supervisor: Dr. James Heubi, (636-8046), email: james.heubi@uc.edu
4. Contacts: Administrative Manager, Ms. Andrea Smith, (636-4273); Research Coordinator, Ms. Karen Schulte, (636-4412)

Hoxworth

Cellular Therapies Division Laboratory

1. Purpose: Stem Cell Processing/Cryopreservation and Laboratory support for Transplant Programs
2. Location: Hoxworth, Room 5016, ML# 0055 (Lab Phone 558-1551 - Office Phone 558-1553)
3. Faculty Supervisor: Tom Leemhius, Ph.D. Office Phone: 558-1553 Manager: Teri Crouch MT, ASCP, Email: crouchtj@ucmail.uc.edu, (Office Phone: 558-1552)
4. Fee Structure: Fee for service
5. Frequency of use: 5% internal use - 95% external use
6. Accessible to all bone marrow/solid organ transplant programs

Imaging Research Center

1. Primary purpose(s) and capabilities: Magnetic Resonance Imaging, multinuclear magnetic Resonance Spectroscopy, x-ray imaging, fluoroscopy and angiography, ultrasound imaging and doppler flow imaging. These facilities are available for research using phantoms and animal models. A 3.0 tesla, magnetic resonance scanner is available for both animal and human investigations at high field.
2. Location: Department of Radiology, CHMC/CHRF, R Level, ML# 0054
3. Director: Bernard Dardzinski, Ph.D., 513-636-7721, Email bernard.dardzinski@cchmc.org
4. Fee structure: Funded by the Departments of Radiology and Pediatrics and from external research grant funds. Call Director for more information
5. Frequency of use: call Director for information
6. Accessible to outside faculty
7. Web site http://www.cincinnatichildrens.org/research/cores/irc/

Inhalation

1. Purpose: animal exposures for inhalation hazards and aerosols
2. Location: Department of Environmental Health, Kettering, Rm. 267, ML# 0056
3. Faculty Supervisor: Dr. George Leikauf, (558-0039), email: leikaugd@ucbeh.san.uc.edu
4. Fee structure: Open
5. Frequency of use: 50% departmental users, 50% no-departmental users
6. Accessible to all departments

Nuclear Magnetic Resonance

1. Purpose: Determination of solution structures and structural dynamics of macromolecules
2. Location: Department of Molecular Genetics, Biochemistry and Microbiology MSB 2105A, ML# 0524
3. Faculty Supervisors: Dr. Paul Rosevear (8-3370) email: rosevear@proto.med.uc.edu, Dr. Mark Rance (8-0066) and Jack Howarth (8-4420)
4. Fee structure: Work will either be collaborative with Drs. Rosevear and Rance, or will require a user contribution to operation of the facility
5. Frequency of use: When installed - 24 hours/day and seven days/week
6. Accessible: Limited

Oligonucleotide Synthesis

1. Purpose: Make oligos
2. Location: Department of Pediatrics, CHMC/CHRF,Room 4006, ML# 0054
3. Faculty Supervisor: Cindy Bachurski, Ph.D. (636-8120), email: cindy.bachurski@cchmc.org Dr. Jeffrey Whitsett, Director (636-7665)
4. Fee structure: Fee for service
5. Frequency of use: 99% internal
6. Accessible to anyone

Radiochemistry

1. Primary purpose(s) and capabilities: Synthesis of new radiopharmaceuticals for preclinical and clinical studies
2. Location: Department of Radiology, 301-311 Old Shriner's Building, ML# 0577
3. Faculty Supervisor: Harry Maxon, M.D.; Day-to-Day Director, Lee Washburn, Ph.D. (8-9049)
4. Fee structure: Funded by the Department of Radiology and external research grants for specific projects.
5. Frequency of use: Five to seven days/week, 80% department, 20% outside
6. Accessible to outside faculty

Expression Technology

BioInformatics*

1. Purpose: The BioInformatics Core provides access to computer systems, database infrastructure, and direct user help for the characterization of genes and gene groups that exhibit coordinate regulation and cooperative function across the biological models under study in the Cincinnati research community. The core seeks to exploit the extraordinary insights being gained from large-scale genome sequencing projects for application to diverse basic and clinical research activities. The principle areas of focus are in gene sequence analysis and gene expression pattern analysis. Also housed here is the development of genomic and biologic databases.

The BioInformatics Core

• aids investigators in the design and analysis of complex experiments that will shed light into the molecular basis of biological processes both through user training and focused project support,
• aids the microarray, cDNA library, and proteomics cores in the organization and management of tracking and reference data for gene clones, PCR products, protein samples and gel images,
• generates a web accessible database that will allow public access to gene expression data, and analyses, from all components, and
• promotes the design and execution of experiments that allow multiple research groups to synergize in their efforts.
2. Location: Children's Hospital Medical Center, New Research Tower - Room 8505, ML#0054
3. Faculty Directors:
Genomics, BioInformatics, Proteomics Bruce Aronow, PhD, 636-4865, email: bruce.aronow@cchmc.org Informatics:John Pestian, PhD, 636-2051,email: jpestian@cchmc.org
4. Equipment: Genome Server The Genome server is a Sun Enterprise 3500 with four UltraSPARC II processors. It has 4 GB RAM and 500 GB hard drive storage. Currenty, Genome houses our relational databases. E450 The E450 is a Sun Enterprise 450 with four UltraSPARC II 400 MHZ processors. It has 2 GB RAM and 108 GB hard drive storage. The E450 acts as the Java Server for various software programs. Currently, the E450 runs Tomcat Java Server and is home to our gene expression database web server, which is near completion. This is an implementation of GeNet from Silicon Genetics. GEMTools Server The GEMTools server is a bioinformatics application server available to all UC/CHMC investigators for the analysis of microarray expression data. It is a Windows NT® 4.0 terminal server running Citrix MetaFrame 1.8 that requires users to install a specific mini-application on their laboratory computer that allows them to login to GEMTools and gain access to software applications such as Incyte GEMTools, SiliconGenetics GeneSpring, Affymetrix GeneChip®, SPSS genomics and statistical analysis programs as well as Microsoft Office. The GEMTools server is a Hewlett Packard® NetServer LH 4r with four Pentium® II Xeon processors and 2 GB of SDRAM and a 100GB drive array backed up daily. RES_SQL Server The RES_SQL server is the database backend to the GEMTools server. Its primary purpose is to act as a Microsoft® SQL server, for the GEMTools application. RES_SQL also acts as an ARCserve®IT backup server, providing an excellent method of backup and recovery. Like its counterpart, RES_SQL is also a Hewlett Packard NetServer LH 4r, with four Pentium II Xeon processors. Test Servers A mirror of all equipment allows for complete testing of applications and upgrades before deployment on our production servers. Sun T3 Storage Array Data are stored on our T3 Storage array. Connected through gigabit ethernet, data can be quickly accessed and manipulated. Currently two terabytes of data storage is available to Cincinnati Children's Research Foundation investigators.
5. Fee Structure: We do not have a for-service fee model in place at this time. The genomics and bioinformatics core is particularly interested in collaborative studies that will contribute to the development of integrated genetic, biologic, and disease model database resource development. Thus, grant project relationships are the best approach.
6. Frequency of use: The facilities are being used intensively.
7. Accessibility: Always available online. Sign up for an account today.
8. Web Page Links: http://genome.uc.edu/ http://info.chmcc.org CEG (Center for Environmental Genetics) Bioinformatics: http://www.uc.edu/bioinformatics
9. Instructional courses are scheduled regularly. See the websites for details.

Genomics

1. Purpose: The Genomics Core facility, known as the Genomics and Microarray Laboratory (GML, was established to perform imcroarray services for UC and CHMC investigators. The services include,
   • microspotting of commercial and custom DNA libraries
   • target labeling and hybridization
   • scanning and data analysis
   • provide advice on methodologies and controls
   • serve as a repository for libraries, amplified DNAs, and microarray slides
   • teaching technologies and establishing an "Empwer The User" program
2. Location: Kettering Lab 336
3. Equipment: In addition to general laboratory hardware, the GML contains specialized genomics equipment, including a 32-pin Gene Machines microarrayer, a Tetrad MJ Research Inc. thermocycler for 96-well and 384-well plates, two BioRobotic Qiagen 3000 robots dedicated to liquid dispensing and DNA isolation and purification, and a dual laser Axon Instruments fluorescent slide scanner. In addition, several networked computers and bar coding devices play key roles in the error-free flow of information from clone plates to microarrays to databases.
4. Faculty Supervisors: Dr. Alvaro Puga, Co-Director 558-0916 Kettering Lab 424 Alvaro.Puga@uc.edu Dr. Craig Tomlinson, Co-Director 558-4769, Kettering Lab 355 Craig.Tomlinson@uc.edu
5. Fee Structure:

Prices for UC/CHMC Investigators		Entire Mouse or Human Library arrays for Triplicate Experiment
Products and Services	Cost/Slide ($)	MouseCost/3 Slides ($)	HumanCost/9 Slides ($)
Microarrayed Slide only with no service	82.00	-	-
Target Labeling	69.00	-	-
Hybridization	24.00	-	-
Scanning and Data Analysis	47.00	-	-
Full Service (all the above)	182.00	499.00	1,497.00
Clone Order	7.00	-	-

6. Frequency of Use:
   95% internal 5% external
7. Accessible Monday through Friday, 8:00AM - 5:00PM. For further information and ordering, go to our web site.
8. Web Page Link: http://microarray.uc.edu

Flow Cytometry

Flow Cytometry

1. Purpose: Analytical flow cytometry of cells in suspension; no sorting capability
2. Location: Department of Internal Medicine - Hematology/Oncology, Division of Hematology/Oncology, Room K-313, ML# 0528
3. Faculty Supervisor: Dr. Robert Franco, (558-3241), email: robert.franco@uc.edu
4. Fee structure: Users contribute to support of instrument proportional to use
5. Frequency of Use: 10-15 hours per week
6. Accessibility is first priority within Cardiovascular Biomaterials Consortium, but as throughput is high, time is available to others

Flow Cytometry/Cell Sorting Laboratory

1. Purpose: To offer cell sorting and analysis capability and expertise to the entire clinical research community
2. Location: CHMC/TCHRF Room 1314 (Dan Marmer 636-8614, or Susan Lee 559-4769), ML# 0054
3. Faculty Supervisor: Darryl Ann Hake; Facilities Manager: Dan Marmer, email: marmd0@cchmc.org
4. Fee structure:
   Sorting: Sterile-$85 set-up charge; $85/hour run time; 1 hour minimum charge; Cell Analysis: $45/hour; Data Analysis: $25/hour
5. Frequency of use: 75% internal/25% external (historical, not mandated)
6. Accessible Monday through Friday, 7:30A - 5:00P

Heart

Small Animal Heart Cellular Electrophysiology, Pharmacology and Physiology

1. Purpose: Consult and carry out contemporary pharmacology, physiology and cellular electrophysiology studies on isolated preparations.
2. Location: Cardiovascular Research Center: G-940-959
3. Faculty Supervisor: Dr. Arnold Schwartz, 558-2400; schwara@ucmail.uc.edu; Associate: Dr. Natasha N. Petrashevskaya; petrasnn@ucmail.uc.edu
4. Types of Preparations: Isolated heart preparations, single cardiac cell and various other cell types with molecular genetic modifications. Computerized technology employed.
5. Some studies available: Quantitative contraction and relaxation parameters, heart rate, coronary flow, Frank-Starling relationships, force-frequency, complete pharmacological profiles, analyses of transgenic models of cardiac hypertrophy/failure, ischemic reperfusion protocols, etc. Patch-clamp, whole cell and single channel analysis, Ca²⁺, Na⁺, K⁺ and Cl^-
6. Fee Structure: Generally no charge or negotiable if desired. Purpose is to assist with pilot data for grants and develop collaborations.
7. Frequency of Use: 100% Medical Center.
8. Web Page Link: http://www.med.uc.edu/heart_core/default.html
9. Training available and encouraged.

Mass Spectrometry

Clinical Mass Spectrometry Center

1. Purpose: Analysis for steroid, bile acid and related compounds
2. Location: CHMC/New Research Building NRB R030 (636-4203), ML# 0054
3. Faculty Supervisor: Dr. Kenneth D. R. Setchell, email: setck0@cchmc.org
4. Fee structure: $350.00 per sample for bile acid screening of potential inborn error
5. Frequency of use: 80% external/20% internal
6. Accessible to all

Mass Spectrometry Facility

Microscopy

Electron Microscopy

1. Purpose: Preparation and examination of ultrathin sections
2. Location: Department of Pathology and Laboratory Medicine, Room 1204 MSB, ML# 0529
3. Faculty Supervisor/Director: Dr. Tito Cavallo, (558-6476), email: tito.cavallo@uc.edu
4. Fee structure: Users "contribute" to support
5. Frequency of use: Sometimes heavy, but scheduling has not been a problem
6. Accessible to all

Live Microscopy Core

1. Purpose:  The facility has two advanced light microscopes optimized for work with living cells and tissues.  All data is automatically backed up after every user session to a remote computer that allows users password-controlled access to archived data for a month.
2. Location: Department of Molecular & Cellular Physiology, MSB 3155, ML #0576
3. Equipment:
   1. Zeiss LSM510 META confocal microscope
      1. Available all mornings and weekends by arrangement
      2. Microscope:  Zeiss Axiovert 200 inverted microscope platform
      3. Microscope objectives: 40X C-Apochromat (water immersion), 10X C-Apochromat (water immersion)
      4. Two conventional confocal detectors
      5. META detector that allows spectral analysis of emission over the range of 460-800 nm with roughly 10 nm resolution
      6. Transmitted light detector for laser-light Nomarski (differential interference contrast) imaging
      7. Fluorescence filter blocks for direct visualization of double and triple labeled samples by eye
   2. Zeiss LSM510 NLO two-photon and confocal microscope
      1. Available 24/7
      2. Microscope: Zeiss Axiovert 200 inverted microscope platform
      3. Dark Environmental chamber: encloses microscope to allow 37°C control and keep stray light out of detecton
      4. Microscope objectives: 40X C-Apochromat (water immersion), 10X C-Apochromat (water immersion)
      5. Lasers for excitation of fluorescence: Ar (458, 477, 488, and 514 nm), green HeNe (543nm) and red HeNe (633 nm), Ti-Sa (700-990 nm).
      6. Two non-descanned detectors that allow collection of light from deep tissue two-photon imaging with higher efficiency than confocal detectors.
      7. Transmitted light detector for laser-light Nomarski (differential interference contrast) imaging
      8. Fluorescence filter blocks for direct visualization of double and triple labeled samples by eye
4. Faculty Supervisors:  Manager, Ms. Jin Dong and Director, Marshall Montrose, Ph.D. 558-5636 or email mhm@uc.edu.
5. Fee Structure:  $250/person user training for novices. $100/person two-photon training and “entrance exam” for users familiar with Zeiss LSM. $25/hour usage fee on either microscope M-F 8-5. $15/hour usage fee on either microscope off-hours and weekends. CD-R or CD-RW provided at cost to users to facilitate data storage.
6. Accessible to:  The facility is self-serve, open only to users who have undergone full training so that they can work independently. Card key access provides 24/7 access to the facility for trained users.
7. Web page link:  http://mcp.uc.edu/index.php?level2=catalog&level3=item_list&product_category_array[0]=1&product_category_array[1]=4

The Center for Biological Microscopy

1. Purpose: Our goal is to assist the researcher in generating high-resolution, high quality microscopy-based data for publications and presentation at professional venues. A range of services is available for both experienced and inexperienced users. Experienced users may use the Core's instruments after proper orientation by a staff member. Inexperienced users may choose to receive training in the use of the instruments, technical support in microscopy and image analysis, consultation in experimental design, or have us perform the microscopy for them as a service.
2. Locations: Dept. of Cell Biology, Neurobiology, and Anatomy, ML #0521, Vontz Center for Molecular Biology, Rms. 3209, 3403 and 3432.
3. Equipment:
   1. Confocal microscopy (Vontz 3403B). The Zeiss LSM 510 laser scanning confocal microscope can be fitted to either an upright or inverted scope. Images for three dyes plus a transmitted, DIC image can be acquired. Scope includes 3-D software, multi-tracking, separate pinholes for each channel, and exquisite control over laser's region of interest. Laser lines: 458, 488, 514, 543, 633 nm. Live cells may be imaged using the inverted platform and data analyzed with the LSM physiology software. Stage and objective heaters aid live cell imaging.
   2. Widefield microscopy.
      1. OrcaERZeiss (Vontz 3403) - A Zeiss Axioplan Imaging 2 infinity-corrected, upright scope with DIC and epifluorescence is coupled to a super sensitive, cooled CCD camera (Orca-ER). Filters are available for several dyes: DAPI, CFP, FITC/YFP, Rhodamine, Texas Red, Cy5.
      2. Spotcam1 (Room 3403) - A SpotII, cooled CCD camera is coupled to a Nikon Microphot upright scope that is set up for epifluorescence, darkfield, and brightfield.
      3. Spotcam2 (Room 3403A) - A SpotII, cooled CCD camera is coupled to a Nikon Microphot upright scope that is set up for epifluorescence, darkfield, brightfield, and phase contrast.
      4. Live Cell Imaging (Room 3403) - Multiple instruments are equipped for live cell imaging ranging from routine phase contrast, time-lapse recording to FRAP (fluorescence recovery after photobleaching) and time-lapse fluorescence imaging of GFP-linked molecules.
      5. Eppendorf Automatic Microinjection system. (Room 3403A)
      6. 35mm Digital camera (Room 3432) - The Nikon D1x is a 35mm, single lens reflex camera with 3008 x 1960 pixels and a 105 mm lens. It can be attached to our Nikon inverted scope to obtain phase or fluorescent images of live cells or attached to our copy stand for digitizing EM negatives and for macroscopic imaging (2-D gels, organs).
   3. Transmission electron microscopy (TEM) (Room 3209). The electron microscopy facility maintains a JEOL JEM-1230 instrument fitted with an AMT Advantage Plus 2K x 2K digital camera, as well as a fully equipped sample processing laboratory, darkroom, and equipment for converting film images to digital images. The facility offers complete EM services including sample processing, ultramicrotomy, immunogold labeling, TEM imaging, film developing, photographic printing, and digital image acquisition. We stress user-friendliness and welcome users who wish to be trained in any or all aspects of electron microscopy.
   4. Image analysis and output. Metamorph (Industrial-strength image analysis software for morphometry, cell counting, intensity quantification, and colocalization), AutoDeblur (deconvolution software to remove out of focus blur), 3D for LSM (measurement and manipulation of 3D image sets), NIH Image, Adobe Photoshop. Fujix Pictography 3000 (photo-quality printer), Tektronix Phaser 850 (photo quality, wax-transfer printer), Nikon Super Coolscan 4000 film scanner, Montage FR2 slide maker, HP Scanjet 7400C.
4. Faculty Supervisors: For training, service and consultation in electron microscopy and confocal microscopy, contact Christina at 513-300-4801, via email, or at the Vontz Center in Room 3209. For training and consultation in widefield microscopy and digital imaging, contact Nancy at 513-300-4803, via email, or at the Vontz Center in Room 3106. Faculty contact: Wallace Ip, Ph.D. 513-558-3614, email)
5. Fee structure: Confocal microscope is $50/hr, EM is $40/hr, OrcaERZeiss is $15/hr, Spotcam systems are $5/hr. Fees for other service (e.g., sample preparation, time-lapse) are listed at our website (LM fees/EM fees). Training is free.
6. Frequency of use:
   Widefield: medium (used >50% time) Confocal, heavy (used >75% time) TEM, frequent (used 50% to 75% time)
7. Accessible to all researchers at UC. We also serve outside users but at slightly higher rates.
8. Web Page Link: microscopy.uc.edu This website has equipment descriptions, contact info, new user application form, reservation procedures, illustrated protocols, image galleries, links & downloads, and a publication list.

Schubert Electron Microscopy

1. Purpose: Ultrastructural examination of tissue
2. Location: Department of Pathology, 4^th Floor Tower, CHMC, (636-4261) ML# 0054
3. Technical Supervisor: Pam Groen (636-8159)
4. Fee structure: Negotiable
5. Frequency of use: 75% departmental diagnostics/25% research
6. Accessible to anyone in CHMC/Research Foundation, training required, technical advice and support available. For individuals outside CHMC, availability depends on collaboration with faculty at CHMC

Mouse

Gene-Targeted Mouse Service

1. Purpose:
   1. Targeting of mouse genes in embryonic stem (ES) cells for gene deletions or mutations (University of Cincinnati only)
   2. Microinjection of targeted ES cells into mouse blastocyst for the production of mice carrying gene deletions or mutations (University of Cincinnati and outside)
2. Location: Department of Molecular Genetics, Biochemistry and Microbiology, Room 2151 MSB and Room 2928 CVC, ML# 0524
3. Faculty Supervisor and Director: Dr. Thomas Doetschman (8-0090) email: Thomas.Doetschman@uc.edu
4. Fee structure: User fees
5. Frequency of use: University of Cincinnati and, in the case of microinjection procedures, University of Cincinnati and academic investigators nation and worldwide
6. Accessible to all funded investigators
7. Web Page Link: http://molgen.uc.edu/logic/

Mouse Pulmonary Function

1. Purpose: Non-invasive measurements in conscious mice
2. Location: Department of Environmental Health, Kettering, Room 267, ML# 0056
3. Faculty Supervisor: Dr. George Leikauf, (558-0039), email: leikaugd@ucbeh.san.uc.edu
4. Fee structure: Undetermined
5. Frequency of use: Daily
6. Accessible to all University faculty

Small Animal Heart Cellular Electrophysiology, Pharmacology and Physiology

1. Purpose: Consult and carry out contemporary pharmacology, physiology and cellular electrophysiology studies on isolated preparations.
2. Location: Cardiovascular Research Center: G-940-959
3. Faculty Supervisor: Dr. Arnold Schwartz, 558-2400; schwara@ucmail.uc.edu; Associate: Dr. Natasha N. Petrashevskaya; petrasnn@ucmail.uc.edu
4. Types of Preparations: Isolated heart preparations, single cardiac cell and various other cell types with molecular genetic modifications. Computerized technology employed.
5. Some studies available: Quantitative contraction and relaxation parameters, heart rate, coronary flow, Frank-Starling relationships, force-frequency, complete pharmacological profiles, analyses of transgenic models of cardiac hypertrophy/failure, ischemic reperfusion protocols, etc. Patch-clamp, whole cell and single channel analysis, Ca²⁺, Na⁺, ⁺ and Cl^-
6. Fee Structure: Generally no charge or negotiable if desired. Purpose is to assist with pilot data for grants and develop collaborations.
7. Frequency of Use: 100% Medical Center.
8. Web Page Link: http://www.med.uc.edu/heart_core/default.html
9. Training available and encouraged.

Transgenic Mice

Transgenic Mouse Core

1. Purpose: Production of transgenic mice bearing exogenous genes
2. Location: Department of Molecular Genetics, Biochemistry and Microbiology, Room 2002 MSB, ML# 0524
3. Faculty Supervisor: Dr. Jerry Lingrel (8-5324); Director: Jon Neumann (8-5294), email: neumanjc@ucbeh.san.uc.edu
4. Fee structure: Supported by funds from the POE, MMD and user fees
5. Frequency of use: Used full-time; roughly 50-50 division between internal and external
6. Accessible to all funded investigators
7. Web page link: http://www.med.uc.edu/htdocs/medicine/trans_web/home.html

Transgenic Mouse Laboratory

1. Purpose: Generation of genetically altered mice and rabbits; embryo cryopreservation
2. Location: Department of Pediatrics CHMC/CHRF, TCHRF Room 3018A, ML# 0054
3. Faculty Supervisor: Dr. S. Potter (636-4851); Operations: Dr. Karen Yager (636-8733), email: yagek0@cchmc.org
4. Fee structure: User charges
5. Frequency of use: Currently CHRF 80%; UC 15%; other 5%
6. Accessible to faculty from outside CHRF

Transgenic Mouse Physiology Core

1. Purpose: Analysis of a variety of physiological (cardiac, renal, pulmonary) functions in intact transgenic mice and development of new technologies/instrumentation related thereto.
2. Location: Department of Molecular and Cellular Physiology, MSB 4056, ML# 0576
3. Faculty Supervisor: Dr. John Lorenz (558-3097), email: john.lorenz@uc.edu
4. Fee structure: Either joint grants or user fees; presently derives some fixed-term support from the Dean’s office
5. Frequency of use: 20% Physiology Department, 20% CHRF and 60% other departments
6. Accessible to all

Tissue Bank

Tissue Culture Laboratory

1. Primary Purpose: Clinical and Research Service. Establish cell lines, immortalize lymphoid lines - storage facility
2. Location: CHMC/TCHRF, Room 1012A (636-4474), ML# 0054 Human Genetics
3. Faculty Supervisors: Dr. Howard Saal and Ruthann Blough, Ph.D., email: blour0@cchmc.org
4. Fee structure: Fee for service/collaborative arrangements
5. Frequency of use: Approximately 600 samples/year
6. Accessible: Sample referral, arrangements through supervisors

Tissue Procurement

1. Purpose: Provides frozen tissue samples in cryovials
2. Location: Department of Pathology and Laboratory Medicine, MSB 1255, ML# 0529
3. Faculty Supervisor: Dr. Sue Heffelfinger, (8-1795); Operations: Gary DeVoe, (8-4675), email: gary.devoe@uc.edu
4. Fee structure: None
5. Frequency of use: This is a new facility whose services may be expanded. Individuals with specific suggestions for added capabilities are urged to contact Dr. Heffelfinger, or a member of the advisory committee (Drs. Stambrook, Groden, Zaknoen and Arceci)
6. Accessible to all

Viral Vector Core

Viral Vector Core

1. Purpose: The Viral Vector Core (VVC) is a joint effort of the Divisions of Experimental Hematology and Pulmonary Biology and provides research-grade Retroviral and Adenoviral Vectors based on a fee-for-service model to support and enhance research at the Cincinnati Children's Hospital, the University of Cincinnati Medical Centers, the Genome Research Institute and the Cincinnati Children's Hospital Research Foundation. Services can be requested by faculty or staff members.

2. Location: Research-grade Retroviral and Adenoviral vectors are produced in physically separated, dedicated Biosafety Level 2 (BL2) laboratories. Retroviral Vectors are produced in the Vector Production Facility (VPF), located on the third floor of the Hoxworth Building or in the Cincinnati Children's Hospital Research Building under the direction of Han van der Loo, PhD. Adenoviral vectors are prepared in a BL2 laboratory located on the 4th floor of the Research Building directed by Bruce Trapnell, MD.
3. Faculty Directors:
The Viral Vector Core and Retroviral Vector component of the Core are under the direction of Dr. Han van der Loo, Division of Experimental Hematology, Department of Pediatrics ( Han.vanderloo@cchmc.org ). Ph 513-475-4093 The adenoviral vector component of the core is managed by Dr. Bruce Trapnell, Division of Pulmonary Biology, Department of Pediatrics ( Bruce.Trapnell@cchmc.org ). Ph 513-636-6361
4. Equipment/Services: The VVC began operations in the fall of 2003 and has since produced 120 different viral vector products. We are offering the following research level services: 1. Production of retroviral and adenoviral vectors using vector plasmids provided by the investigator. 2. Generation of stable retroviral producer cell lines. 3. Vector titration. 4. Mycoplasma and sterility testing. 5. Plasmid purification. 6. Vector concentration (select envelopes). 7. Lentiviral vector generation and RCL testing. (to be available in Fall of 2004.)

5. Fee Structure: The costs for services are charged to the Division's or Department's budget through the CCHMC Research Administration Service Center. Overall, fees have been subsidized to allow cost efficiency to investigators. Contact the VVC for a pricing overview or for a formal Quote.
6. Accessibility: Investigators requiring Vector Core services should complete and submit a Request Form for Retroviral or Adenoviral Vector. Request forms can be downloaded from the Vector Core website at http://www.cincinnatichildrens.org/research/div/exp-hematology/translational/vpf/vvc/default.htm
For general information regarding the Viral Vector Core, or for further information regarding the Retroviral Vector component of the Core, please contact Dr. Han van der Loo at 513-475-4093. For further information regarding the Adenoviral Vector component of the Core, please contact Dr. Bruce Trapnell at 513-636-6361.
7. Webpage link: http://www.cincinnatichildrens.org/research/div/exp-hematology/translational/vpf/vvc/default.htm