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Research Core Facilities

Core Facilities

Expression Technology

    Biomedical Informatics / Research IT Services

    1. Purpose:  Provides computational resources, services and support to investigators at Cincinnati Children’s Research Foundation and the University of Cincinnati College of Medicine; serves as the initial point of contact for all inquiries about hardware, software and other information technology related to research; provides strategic guidance on the impact of computation systems and data on research.

    2. Location:  Cincinnati Children's Hospital Medical Center
      ML#7024

    3. Director:  John Hutton, MD
      Phone: 513-636-0265
      john.hutton@cchmc.org

    4. Fee structure:  For details, contact
      Eszter Belcher
      eszter.belcher@cchmc.org
      513-636-0148

    5. Accessible:  Cincinnati Children’s and UC College of Medicine investigators

    6. Website:  http://bmi.cchmc.org


    Genomics

    1. Purpose:  The Genomics and Microarray Laboratory (GML) has been established to perform one-stop genomics and microarray services for UC and CHMC investigators. We provide a complete package of products and services comprising of a variety of microarrays, optimal experimental design, and exhaustive statistical and bioinformatic analysis. We provide both Affymetrix and 2-color printed microarray products.  Some of the specific microarray products we offer include: Affymetrix microarrays, in-house whole genome microarrays of rat, human, and mouse; miRNA, CGH, and promoter microarrays; RNA quality testing; custom microarray printing; and amplification of small RNA samples extracted from LCM samples and various other sources.  These services are continually being expanded and the most updated listing can be found on our website microarray.uc.edu.

    2. Location:  Kettering Lab 336

    3. Director:  Dr. Shuk-Mei Ho
      Email: microarray@uc.edu
      Phone:513-558-4764

    4. Fee structure:  Please go to our website (microarray.uc.edu) to view the most current pricing.

    5. Frequency of use:  Approximately 95% internal 5% external.

    6. Accessible:  Monday through Friday, 9:00AM - 5:00PM. For further information and ordering, go to our web site.

    7. Website:  http://microarray.uc.edu


    Proteomics

    1. Purpose: 
      Our mission is provide expertise & coordinate services in proteomics and biological mass spectrometry to the UC & CCHMC research community.  This includes both fee-for-service projects and collaborative research projects.

    2. Location: 
      Proteomics Laboratory
      GRI, Building B, Rooms 126-131
      Lab Phone:  513-558-4057
      Director Phone: 513-558-7102
      ML 0505

    3. Equipment: 
      Mass Spectrometers
      (1)     AB 4800 MALDI-TOF/TOF MS w/Tempo nanoLC
      (2)     Thermo LTQ linear Ion-Trap-MS w/Eksigent nanoLC
      (3)     ThermoLCQDeca XP Ion-Trap-MS w/Dionex nanoLC
      (4)     MicroMass/Waters LCT (LC-ESI-TOF-MS) 

      2-D gel equipment
      (1)     Four Amersham Multiphor II isoelectric focusing systems
      (2)     Four Genomic Solutions Investigator 2-D system
      (3)     Compugen Z3 Gel Analysis Imaging System
      (4)    GE Healthcare (Amersham) DIGE 2-D gel analysis system
      (5)     Fuji Fluorescence scanner


    4. Director: 
      Kenneth D. Greis, Ph.D
      Associate Professor
      Department of Genome Science
      Genome Research Institute
      2180 E. Galbraith Road, Rm B109
      Cincinnati,OH 45237
      513 558-5061 Fax
      513 558-7102
      email: ken.greis@uc.edu

      Lab Personnel
      Wendy Dominick, Ph.D.
      Mass Spectrometry
      Phone: 513-558-4057

      Hongjun Shu, Ph.D.
      Mass Spectrometry
      Phone: 513-558-4057

      Mike Wyder, M.S
      2-D gel separation, Image analysis, Mass Spectrometry
      Phone: 513-558-4057

      Kelly Rask, M.S
      Mass Spectrometry, 2-D gel separation, Image analysis
      Phone: 513-558-4057

    5. Fee structure: 

      Proteomics Services

      cost per item

      1.  In gel digestion and recovery

      $20

      2.  MALDI-TOF/TOF protein ID

      $49

      3.  LC-MS/MS Protein ID

      $131

      4.  LC-MALDI-TOF/TOF

      $163

      5.  MALDI-TOF of intact protein

      $54

      6.  LC-ESI-TOF of proteins

      $64

      7.  Serum Immunodepletion

      $70

      8.  Protein Concentration Assay

      $9

      9.  DIGE labeling per Rxn pair

      $260

      10. 2D gel separation

      $111

      11. Gel Stain (Silver/Coomassie)

      $17

      12. Gel stain (Sypro/ProQ Diamond)  

      $76

      13. Fluorescent Image Capture

      $41

      14. Image analysis per hour

      $30

      15. Others Services

       

            (Time and material costs)

      Contact the Director


    6. Frequency of use:  50% UC, 50% CHMC

    7. Accessible:  Monday through Friday, 7:00 AM to 6:00 PM. Call the Director to schedule an appointment for a consultation or for a quotation for on services.

    8. Website:  http://www.gri.uc.edu/extended_info.php?c2h_id=3&extended=Proteomics


Flow Cytometry

    Cell Sorting Laboratory

    1. Purpose:  To offer cell sorting and analysis capability and expertise to the entire clinical research community

    2. Location:  CHMC/TCHRF Room 1314
      Dan Marmer 513-636-8614or Susan Lee 513-559-4769
      ML# 0054

    3. Director:  Darryl Ann Hake

      Facilities Manager
      Dan Marmer
      Email: marmd0@cchmc.org

    4. Frequency of use:  75% internal/25% external (historical, not mandated)

    5. Accessible:  Accessible Monday through Friday, 7:30A - 5:00P



    Flow Cytometry

    1. Purpose:  Analytical flow cytometry of cells in suspension; no sorting capability

    2. Location:  Department of Internal Medicine 
      Hematology/Oncology, Division of Hematology/Oncology
      Room K-313
      ML# 0528

    3. Director:  Dr. Robert Franco
      Email: robert.franco@uc.edu
      Phone: 513-558-3241

    4. Fee structure:  Users contribute to support of instrument proportional to use

    5. Frequency of use:  10-15 hours per week

    6. Accessible:  Accessibility is first priority within Cardiovascular Biomaterials Consortium, but as throughput is high, time is available to others



General

    Biological Containment

    1. Purpose:  Containment facility for research on Biosafety Level 3 pathogens.

    2. Location:  University of Cincinnati College of Medicine

    3. Director:  Neal Wolfe (558-3486) email: wolfen@ucmail.uc.edu

    4. Fee structure:  Hourly charge

    5. Frequency of use:  Unlimited

    6. Accessible:  Open to all qualified UC and select non-UC personnel



    Cell Manipulation Core
    Digestive Disease Research Development Center (DDRDC)

    1. Purpose:  The DDRDC Cell Manipulation Core provides cells either from primary tissue harvest (liver or intestine) or existing established cell lines (intestinal, liver, and pancreatic) to members of the DDRDC.  This core specializes in modifying gene expression in cell culture. 

    2. Location:  CCHMC Building R (Research Foundation Building) Room 2030

    3. Director:  Jorge A. Bezerra, MD (636-3008) email: jorge.bezerra@cchmc.org

    4. Frequency of use:  Unlimited

    5. Accessible:  Accessible to members of the Digestive Disease Research Development Center

    6. Website:  http://www.cincinnatichildrens.org/research/project/ddrdc/cores/


    Center for Biostatistical Services

    1. Purpose:  Enhance sponsored program activity in the Academic Health Center by providing statistical rigor to the research proposals and programs.

    2. Location:  Room G-27, Kettering Lab, ML #0056

    3. Director:  Rakesh Shukla, Ph.D. , Phone: 513-558-0108; E-mail: Rakesh.Shukla@uc.edu

    4. Fee structure:  All assistance directly towards a grant-proposal being submitted for extramural funding by a college of Medicine faculty is institutionally subsidized and thus is "free". All other services by the core have university approved rates. Please see the WEB SITE for details.

    5. Frequency of use:  See the WEB site listed below.

    6. Accessible:  Yes by anyone and everyone.

    7. Website:  http://www.med.uc.edu/biostatistics


    Comparative Pathology

    1. Purpose:  Consult and carry out pathology studies on animals or animal tissues.

    2. Location:  Medical Science Building 1054 and 1109.

    3. Director:  Dr. Greg Boivin, 558-9156; boivingp@ucmail.uc.edu
      Laboratory Supervisor: Rita Angel and Yvette Doty, 558-4436

    4. Fee structure:  Fee for service based on established schedule. Contact Dr. Boivin for more information. Technical and Professional support can also be added to grants directly.

    5. Frequency of use:  1500 cases are received annually: 60% Medical Center, 25% Children’s Hospital, 10% VA Medical Center, 5% Shriners, West Campus and other Universities.

    6. Accessible:  Accessible and available for sample submission during regular business hours.

    7. Other:  Types of Preparations: Animal necropsy, tissue processing, embedding, sectioning, staining, immunohistochemistry, frozen-sectioning

      Some studies available: Animal necropsy, tissue processing, embedding, sectioning, staining, immunohistochemistry, frozen-sectioning, photographs.

    8. Website:  http://pathology.uc.edu/divisions/comppath.asp


    Cytogenetics/Fish Laboratory

    1. Purpose:  Chromosome analyses/Gene mapping by FISH Clinical and Research Service

    2. Location:  CHMC/TCHRF 1012A (636-4474), ML# 0054, CHRF 1001

    3. Director:  Dr. Howard Saal and Ruthann Blough, Ph.D., email: blour0@cchmc.org

    4. Frequency of use:  Greater than 3,000 samples/year

    5. Accessible:  Sample referral arrangements through supervisors



    Dietary Data Entry Center

    1. Purpose:  Entry and analysis of dietary data including three-day diet records and dietary recalls.

    2. Location:  Children's Hospital Medical Center
      The Research Foundation #7507
      3333 Burnet Avenue
      Cincinnati, Ohio 45229

    3. Director:  Dr. Stephen Daniels:
      E-Mail steve.daniels@cchmc.org
      Phone: 513-636-7272

    4. Frequency of use:  80% outside, 20% inside work

    5. Accessible:  Accessible to anyone needing services

    6. Website:  http://www.cincinnatichildrens.org/chrf/resources/ddec/


    DNA Sequencing and Oligonucleotide Synthesis

    1. Purpose:  Synthesis and purification of oligonucleotides, nucleotide sequence analysis, contig assembly and sequencing template preparation, and mouse and human genotyping

    2. Location:  Department of Molecular Genetics, Biochemistry and Microbiology
      Room 2302 MSB
      ML# 0524

    3. Director:  Dr. Joanna Groden (8-0088);
      Email: joanna.groden@uc.edu
      Director: Doug Bintzler (8-5520)

    4. Fee structure:  Fee for service established; supported by user charges

    5. Frequency of use:  Full-time, about 50% from outside the department of Molecular Genetics

    6. Accessible:  Accessible to all funded investigators



    General Clinical Research Center

    1. Purpose:  Provides facilities and resources for patient-oriented research Biostatistics core/Core Laboratory and Facilities for Bone Related Research

    2. Location:  CHMC, 3rd floor, ML# 0054

    3. Director:  Dr. James Heubi, (636-8046)
      Email: james.heubi@uc.edu



    Hoxworth
    Cellular Therapies Division Laboratory

    1. Purpose:  Stem Cell Processing/Cryopreservation and Laboratory support for Transplant Programs

    2. Location:  Hoxworth Building
      Room 5016
      ML# 0055
      Lab Phone 513-558-1551 - Office Phone 513 558-1553

    3. Director:  Tom Leemhius, Ph.D.
      Office Phone: 513-558-1553

      Manager
      Teri Crouch MT, ASCP
      Email: crouchtj@ucmail.uc.edu
      Phone: 513-558-1552

    4. Fee structure:  Fee for service

    5. Frequency of use:  5% internal use - 95% external use

    6. Accessible:  Accessible to all bone marrow/solid organ transplant programs



    Imaging Research Center

    1. Purpose:  Magnetic Resonance Imaging, multinuclear magnetic Resonance Spectroscopy, x-ray imaging, fluoroscopy and angiography, ultrasound imaging and doppler flow imaging. These facilities are available for research using phantoms and animal models. A 3.0 tesla, magnetic resonance scanner is available for both animal and human investigations at high field.

    2. Location:  Department of Radiology
      CHMC/CHRF, R Level
      ML# 0054

    3. Director:  Bernard Dardzinski, Ph.D.
      Phone: 513-636-7721
      Email bernard.dardzinski@cchmc.org

    4. Frequency of use:  Call Director for information

    5. Accessible:  Accessible to outside faculty

    6. Website:  http://www.cincinnatichildrens.org/research/cores/irc/


    Inhalation

    1. Purpose:  Animal exposures for inhalation hazards and aerosols

    2. Location:  Department of Environmental Health
      Kettering, Rm. 267
      ML# 0056

    3. Director:  Dr. George Leikauf
      Email: leikaugd@ucbeh.san.uc.edu
      Phone: 513-558-0039

    4. Fee structure:  Open

    5. Frequency of use:  50% departmental users, 50% no-departmental users

    6. Accessible:  Accessible to all departments



    Monoclonal Antibody

    1. Purpose:  All aspects of Monoclonal Antibody production

    2. Location:  Department of Pediatrics
      CHMC/CHRF, Room 3047
      ML# 0054

    3. Director:  Dr. James Lessard
      Email: james.lessard@uc.edu
      Phone: 513-636-8308

      Operations
      Sharon Dingle
      Phone: 513-636-8335

    4. Frequency of use:  50% CHMC, 40% UC, 10% remainder outside

    5. Accessible:  Accessible statewide



    Nuclear Magnetic Resonance

    1. Purpose:  Determination of solution structures and structural dynamics of macromolecules

    2. Location:  Department of Molecular Genetics, Biochemistry and Microbiology
      MSB 2105A
      ML# 0524

    3. Director:  Dr. Paul Rosevear
      Email: rosevear@proto.med.uc.edu
      Phone: 513-558-3370

      Dr. Mark Rance
      Phone: 513-558-0066

      Jack Howarth
      Phone: 513-558-4420

    4. Fee structure:  Work will either be collaborative with Drs. Rosevear and Rance, or will require a user contribution to operation of the facility


    5. Frequency of use:  Work will either be collaborative with Drs. Rosevear and Rance, or will require a user contribution to operation of the facility

    6. Accessible:  Limited



    Radiochemistry

    1. Purpose:  Synthesis of new radiopharmaceuticals for preclinical and clinical studies

    2. Location:  Department of Radiology
      301-311 Old Shriner's Building
      ML# 0577

    3. Director:  Harry Maxon, M.D.

      Day-to-Day Director
      Lee Washburn, Ph.D.
      Phone: 513-558-9049

    4. Fee structure:  Funded by the Department of Radiology and external research grants for specific projects.

    5. Frequency of use:  Five to seven days/week, 80% department, 20% outside

    6. Accessible:  Accessible to outside faculty



Heart

    Small Animal Heart Cellular Electrophysiology, Pharmacology and Physiology

    1. Purpose:  Consult and carry out contemporary pharmacology, physiology and cellular electrophysiology studies on isolated preparations.

    2. Location:  Cardiovascular Research Center: G-940-959

    3. Director:  Dr. Arnold Schwartz
      Phone: 513-558-2400
      Email: schwara@ucmail.uc.edu

      Associate
      Dr. Natasha N. Petrashevskaya
      Email: petrasnn@ucmail.uc.edu

    4. Fee structure:  Generally no charge or negotiable if desired. Purpose is to assist with pilot data for grants and develop collaborations.

    5. Frequency of use:  100% Medical Center

    6. Other:  Types of Preparations: Isolated heart preparations, single cardiac cell and various other cell types with molecular genetic modifications. Computerized technology employed.

      Some studies available: Quantitative contraction and relaxation parameters, heart rate, coronary flow, Frank-Starling relationships, force-frequency, complete pharmacological profiles, analyses of transgenic models of cardiac hypertrophy/failure, ischemic reperfusion protocols, etc. Patch-clamp, whole cell and single channel analysis, Ca2+, Na+, K+ and Cl-

      Training available and encouraged.

    7. Website:  http://www.med.uc.edu/heart_core/default.html


Laboratory Animal Medical Services

    Comparative Pathology

    1. Purpose:  Consult and carry out pathology studies on animals or animal tissues.

    2. Location:  Medical Science Building 1054 and 1109.

    3. Director:  Dr. Greg Boivin, 558-9156; boivingp@ucmail.uc.edu
      Laboratory Supervisor: Rita Angel and Yvette Doty, 558-4436

    4. Fee structure:  Fee for service based on established schedule. Contact Dr. Boivin for more information. Technical and Professional support can also be added to grants directly.

    5. Frequency of use:  1500 cases are received annually: 60% Medical Center, 25% Children’s Hospital, 10% VA Medical Center, 5% Shriners, West Campus and other Universities.

    6. Accessible:  Accessible and available for sample submission during regular business hours.

    7. Other:  Types of Preparations: Animal necropsy, tissue processing, embedding, sectioning, staining, immunohistochemistry, frozen-sectioning

      Some studies available: Animal necropsy, tissue processing, embedding, sectioning, staining, immunohistochemistry, frozen-sectioning, photographs.

    8. Website:  http://pathology.uc.edu/divisions/comppath.asp


    Laboratory Animal Medical Services

    1. Purpose: 

      1. To provide a high quality laboratory animal care and use program to the University of Cincinnati research and teaching community.

      2. To serve as a resource to the medial research community through the provision of education, training and consultative services as it relates to laboratory animal care and use.

      3. To provide leadership and direction in response to scientific advancements as it relates to laboratory animal care and use.



    2. Location:  UC ML# 0571

    3. Director: 

      Dr. Douglas W. Stone

      Phone# 513-558-5171



    4. Fee structure:  Per Diem rates and Usage fees

    5. Frequency of use:  7 days per week

    6. Accessible:  Use is limited to UC faculty.

    7. Other: 

      East Campus Animal User's discussion - Wednesday, February 7, 2007

      9:00 – 10:00 AM College of Medicine Room # 3351



      GRI Campus Animal User's Discussion - Thursday, February 1, 2007

      9:30 – 10:30 AM Auditorium Building A



    8. Website:  http://medcenter.uc.edu/LAMS/


Microscopy

    Clinical Mass Spectrometry Center

    1. Purpose:  Analysis for steroid, bile acid and related compounds

    2. Location:  CHMC/New Research Building
      NRB R030
      ML# 0054
      513-636-4203

    3. Director:  Dr. Kenneth D. R. Setchell
      Email: setck0@cchmc.org

    4. Frequency of use:  80% external / 20% internal

    5. Accessible:  Accessible to all



    Comparative Pathology

    1. Purpose:  Consult and carry out pathology studies on animals or animal tissues.

    2. Location:  Medical Science Building 1054 and 1109.

    3. Director:  Dr. Greg Boivin, 558-9156; boivingp@ucmail.uc.edu
      Laboratory Supervisor: Rita Angel and Yvette Doty, 558-4436

    4. Fee structure:  Fee for service based on established schedule. Contact Dr. Boivin for more information. Technical and Professional support can also be added to grants directly.

    5. Frequency of use:  1500 cases are received annually: 60% Medical Center, 25% Children’s Hospital, 10% VA Medical Center, 5% Shriners, West Campus and other Universities.

    6. Accessible:  Accessible and available for sample submission during regular business hours.

    7. Other:  Types of Preparations: Animal necropsy, tissue processing, embedding, sectioning, staining, immunohistochemistry, frozen-sectioning

      Some studies available: Animal necropsy, tissue processing, embedding, sectioning, staining, immunohistochemistry, frozen-sectioning, photographs.

    8. Website:  http://pathology.uc.edu/divisions/comppath.asp


    Electron Microscopy

    1. Purpose:  Preparation and examination of ultrathin sections

    2. Location:  Department of Pathology and Laboratory Medicine
      Room 1204 MSB
      ML# 0529

    3. Director:  Dr. Tito Cavallo
      Email: tito.cavallo@uc.edu
      Phone: 513-558-6476

    4. Fee structure:  Users "contribute" to support

    5. Frequency of use:  Sometimes heavy, but scheduling has not been a problem

    6. Accessible:  Accessible to all



    Live Microscopy Core

    1. Purpose:  The facility has two advanced light microscopes optimized for work with living cells and tissues.  All data is automatically backed up after every user session to a remote computer that allows users password-controlled access to archived data for a month.

    2. Location:  Department of Molecular & Cellular Physiology
      MSB 3155
      ML #0576

    3. Equipment: 
      1. Zeiss LSM510 META confocal microscope
        1. Available all mornings and weekends by arrangement
        2. Microscope:  Zeiss Axiovert 200 inverted microscope platform
        3. Microscope objectives: 40X C-Apochromat (water immersion), 10X C-Apochromat (water immersion)
        4. Two conventional confocal detectors
        5. META detector that allows spectral analysis of emission over the range of 460-800 nm with roughly 10 nm resolution
        6. Transmitted light detector for laser-light Nomarski (differential interference contrast) imaging
        7. Fluorescence filter blocks for direct visualization of double and triple labeled samples by eye
      2. Zeiss LSM510 NLO two-photon and confocal microscope
        1. Available 24/7
        2. Microscope: Zeiss Axiovert 200 inverted microscope platform
        3. Dark Environmental chamber: encloses microscope to allow 37°C control and keep stray light out of detecton
        4. Microscope objectives: 40X C-Apochromat (water immersion), 10X C-Apochromat (water immersion)
        5. Lasers for excitation of fluorescence: Ar (458, 477, 488, and 514 nm), green HeNe (543nm) and red HeNe (633 nm), Ti-Sa (700-990 nm).
        6. Two non-descanned detectors that allow collection of light from deep tissue two-photon imaging with higher efficiency than confocal detectors.
        7. Transmitted light detector for laser-light Nomarski (differential interference contrast) imaging
        8. Fluorescence filter blocks for direct visualization of double and triple labeled samples by eye


    4. Director:  Manager
      Chet Closson
      Email: clossoct@uc.edu
      Phone:  513-558-3791

      Director
      Marshall Montrose, Ph.D.
      Email: mhm@uc.edu
      Phone:  513-558-5636

    5. Fee structure:  $250/person user training for novices. $100/person two-photon training and “entrance exam” for users familiar with Zeiss LSM. $25/hour usage fee on either microscope M-F 8-5. $15/hour usage fee on either microscope off-hours and weekends. CD-R or CD-RW provided at cost to users to facilitate data storage.

    6. Accessible:  The facility is self-serve, open only to users who have undergone full training so that they can work independently. Card key access provides 24/7 access to the facility for trained users.

    7. Website:  http://mcp.uc.edu/index.php?level2=catalog&level3=item_list&product_category_array[0]=1&product_category_array[1]=4


    Schubert Electron Microscopy

    1. Purpose:  Ultrastructural examination of tissue

    2. Location:  Department of Pathology
      4th Floor Tower, CHMC
      ML# 0054
      513-636-4261

    3. Director:  Pam Groen
      Phone: 513-636-8159

    4. Frequency of use:  75% departmental diagnostics / 25% research

    5. Accessible:  Accessible to anyone in CHMC/Research Foundation, training required, technical advice and support available. For individuals outside CHMC, availability depends on collaboration with faculty at CHMC



    The Center for Biological Microscopy

    1. Purpose:  Our goal is to assist the researcher in generating high-resolution, high quality microscopy-based data for publications and presentation at professional venues. A range of services is available for both experienced and inexperienced users. Experienced users may use the Core's instruments after proper orientation by a staff member. Inexperienced users may choose to receive training in the use of the instruments, technical support in microscopy and image analysis, consultation in experimental design, or have us perform the microscopy for them as a service.

    2. Location: 

      Department of Cell and Cancer Biology
      Vontz Center for Molecular Studies
      3125 Eden Avenue, Rooms 3209, 3403 and 3432



    3. Equipment: 
      1. Confocal microscopy (Vontz 3403B). The Zeiss LSM 510 laser scanning confocal microscope can be fitted to either an upright or inverted platform. Images for three dyes plus a transmitted, DIC image can be acquired. Capabilities include multi-tracking, separate pinholes for each channel, exquisite control over the laser's region of interest, and 3-D rendering. Laser lines: 458, 488, 514, 543, 633 nm. Live cells may be imaged using the inverted platform and data analyzed with the LSM physiology software. Stage and objective heaters are available to facilitate live cell imaging.
      2. Widefield microscopy.
        1. OrcaERZeiss (Vontz 3403) - A Zeiss Axioplan Imaging 2 infinity-corrected, upright microscope with DIC and epifluorescence is coupled to a sensitive cooled CCD camera (Orca-ER). Filters are available for DAPI, CFP, FITC/YFP, Rhodamine, Texas Red and Cy5.
        2. Spotcam1 (Room 3403) - A Spot II cooled CCD camera is coupled to a Nikon Microphot upright microscope with epifluorescence, darkfield, and brightfield optics.
        3. Spotcam2 (Room 3403A) - A Spot II cooled CCD camera is coupled to a Nikon Microphot upright microscope with epifluorescence, darkfield, brightfield, and phase contrast optics.
        4. Live Cell Imaging (Room 3403) - Multiple instruments are equipped for live cell imaging ranging from routine phase contrast, time-lapse recording to FRAP (fluorescence recovery after photobleaching) and time-lapse fluorescence imaging of GFP-linked molecules.
        5. Eppendorf Automatic Microinjection system. (Room 3403A)
        6. Professional Digital camera - The Nikon D1x is a professional grade SLR camera with 3008 x 1960 pixels. It can be attached to a Nikon inverted microscope to obtain phase  contrast or fluorescent images of live cells or attached to a copy stand for macroscopic imaging (2-D gels, organs, small animals) and for digitizing EM negatives.
      3. Transmission electron microscopy (TEM) (Room 3209). The electron microscopy facility maintains a JEOL JEM-1230 instrument fitted with an AMT Advantage Plus 2K x 2K digital camera, as well as a fully equipped sample processing laboratory, darkroom, and equipment for converting film images to digital images. The facility offers complete EM services including sample processing, ultramicrotomy, immunogold labeling, TEM imaging, film developing, photographic printing, and digital image acquisition. We stress user-friendliness and welcome users who wish to be trained in any or all aspects of electron microscopy.
      4. Image analysis and output. Metamorph (industry-standard image analysis software for image acquisition, morphometry, cell counting, intensity quantification, and colocalization), AutoDeblur (deconvolution software to remove out of focus blur), 3D for LSM (measurement and manipulation of 3D image sets), ImageJ, Adobe Photoshop. Fujix Pictography 3000 photo-quality printer, Tektronix Phasers, Nikon Super Coolscan 4000 film scanner, Montage FR2 slide maker, HP Scanjet 7400C.


    4. Director:  For training and consultation in electron microscopy, confocal microscopy, widefield microscopy and digital imaging contact Dr. Birgit Ehmer at 513-300-4801, via email, or at the Vontz Center in Room 3104. Faculty director: Dr. Wallace Ip (513-558-3614, email)

    5. Fee structure: 

      Confocal microscope: $30/hr during regular work week, $25/hr after 5 pm and weekends. TEM: $40/hr. OrcaERZeiss: $15/hr during regular work week, $5/hr for time-lapse (after 5 pm and weekends). Spotcam systems: $5/hr. Fees for other service (e.g., sample preparation, time-lapse) are listed at our website ( LM fees/ EM fees). Training is $30/hr.



    6. Frequency of use:  Widefield: moderate (used >50% time)
      Confocal, moderate to heavy (used 50-75% time)
      TEM, light (used <50% time)

    7. Accessible:  Accessible to all researchers in the UC research community including CCHMC and VA. We also serve outside users at slightly higher charges.

    8. Other:  Our website has equipment descriptions, contact information, new user application form, reservation procedures, illustrated protocols, image galleries, links & downloads, and a publication list.

    9. Website:  http://microscopy.uc.edu


Mouse

    Comparative Pathology

    1. Purpose:  Consult and carry out pathology studies on animals or animal tissues.

    2. Location:  Medical Science Building 1054 and 1109.

    3. Director:  Dr. Greg Boivin, 558-9156; boivingp@ucmail.uc.edu
      Laboratory Supervisor: Rita Angel and Yvette Doty, 558-4436

    4. Fee structure:  Fee for service based on established schedule. Contact Dr. Boivin for more information. Technical and Professional support can also be added to grants directly.

    5. Frequency of use:  1500 cases are received annually: 60% Medical Center, 25% Children’s Hospital, 10% VA Medical Center, 5% Shriners, West Campus and other Universities.

    6. Accessible:  Accessible and available for sample submission during regular business hours.

    7. Other:  Types of Preparations: Animal necropsy, tissue processing, embedding, sectioning, staining, immunohistochemistry, frozen-sectioning

      Some studies available: Animal necropsy, tissue processing, embedding, sectioning, staining, immunohistochemistry, frozen-sectioning, photographs.

    8. Website:  http://pathology.uc.edu/divisions/comppath.asp


    Gene-Targeted Mouse Service

    1. Purpose: 
    2. Design and construction of gene targeting vectors
    3. Targeting of mouse genes into embryonic stem (ES) cells for gene deletions or mutations.
    4. Injection of targeted ES cells into mouse blastocyst for the production of mice carrying gene deletions or mutations.


    5. Location: 
      Gene Targeting Mouse Service Core
      University of Cincinnati
      Department of Molecular Genetics, Biochemistry and Microbiology
      Room 2151 MSB and Room 2051 MSB, ML# 0524
      231 Albert Sabin Way
      Cincinnati, OH 45267 


    6. Director: 
      • Dr. Yinhuai Chen (Vector Services)
      • Email: escore@uc.edu
      • Phone: 1-513-558-5314
      • Room Location: MSB 2051
    7. Tina Grisham (Gene Targeting and Blastocyst injection)
    8. Email: escore@uc.edu
    9. Phone: 1-513-4351
    10. Room Location: MSB 2155


    11. Fee structure: 
      Call or email for current price list.


    12. Frequency of use: 
    13. 45% University of Cincinnati and Cincinnati Children's Hospital
    14. 55% Academic investigators nationwide.


    15. Accessible: 
      To all funded investigators


    16. Website:  http://molgen.uc.edu/index.php?level2=corefacilities&level3=corefacilities_gene


    Mouse Pulmonary Function

    1. Purpose:  Non-invasive measurements in conscious mice

    2. Location:  Department of Environmental Health
      Kettering, Room 267
      ML# 0056

    3. Director:  Dr. George Leikauf
      Email: leikaugd@ucbeh.san.uc.edu
      Phone: 513-558-0039

    4. Fee structure:  Undetermined

    5. Frequency of use:  Daily

    6. Accessible:  Accessible to all University faculty



Tissue Bank

    Comparative Pathology

    1. Purpose:  Consult and carry out pathology studies on animals or animal tissues.

    2. Location:  Medical Science Building 1054 and 1109.

    3. Director:  Dr. Greg Boivin, 558-9156; boivingp@ucmail.uc.edu
      Laboratory Supervisor: Rita Angel and Yvette Doty, 558-4436

    4. Fee structure:  Fee for service based on established schedule. Contact Dr. Boivin for more information. Technical and Professional support can also be added to grants directly.

    5. Frequency of use:  1500 cases are received annually: 60% Medical Center, 25% Children’s Hospital, 10% VA Medical Center, 5% Shriners, West Campus and other Universities.

    6. Accessible:  Accessible and available for sample submission during regular business hours.

    7. Other:  Types of Preparations: Animal necropsy, tissue processing, embedding, sectioning, staining, immunohistochemistry, frozen-sectioning

      Some studies available: Animal necropsy, tissue processing, embedding, sectioning, staining, immunohistochemistry, frozen-sectioning, photographs.

    8. Website:  http://pathology.uc.edu/divisions/comppath.asp


    Tissue Culture Laboratory

    1. Purpose:  Clinical and Research Service. Establish cell lines, immortalize lymphoid lines - storage facility

    2. Location:  CHMC/TCHRF
      Room 1012A
      ML# 0054
      Phone: 513-636-4474


    3. Director:  Dr. Howard Saal and Ruthann Blough, Ph.D.
      Email: blour0@cchmc.org

    4. Frequency of use:  Approximately 600 samples/year

    5. Accessible:  Sample referral, arrangements through supervisors



    Tissue Procurement

    1. Purpose:  Provides frozen tissue samples in cryovials

    2. Location:  Department of Pathology and Laboratory Medicine
      MSB 1255
      ML# 0529

    3. Director:  Dr. Sue Heffelfinger
      Phone: 513-558-1795

      Operations
      Gary DeVoe
      Email: gary.devoe@uc.edu
      Phone: 513-558-4675

    4. Fee structure:  None

    5. Frequency of use:  This is a new facility whose services may be expanded. Individuals with specific suggestions for added capabilities are urged to contact Dr. Heffelfinger, or a member of the advisory committee (Drs. Stambrook, Groden, Zaknoen and Arceci)

    6. Accessible:  Accessible to all



Transgenic Animal

    Comparative Pathology

    1. Purpose:  Consult and carry out pathology studies on animals or animal tissues.

    2. Location:  Medical Science Building 1054 and 1109.

    3. Director:  Dr. Greg Boivin, 558-9156; boivingp@ucmail.uc.edu
      Laboratory Supervisor: Rita Angel and Yvette Doty, 558-4436

    4. Fee structure:  Fee for service based on established schedule. Contact Dr. Boivin for more information. Technical and Professional support can also be added to grants directly.

    5. Frequency of use:  1500 cases are received annually: 60% Medical Center, 25% Children’s Hospital, 10% VA Medical Center, 5% Shriners, West Campus and other Universities.

    6. Accessible:  Accessible and available for sample submission during regular business hours.

    7. Other:  Types of Preparations: Animal necropsy, tissue processing, embedding, sectioning, staining, immunohistochemistry, frozen-sectioning

      Some studies available: Animal necropsy, tissue processing, embedding, sectioning, staining, immunohistochemistry, frozen-sectioning, photographs.

    8. Website:  http://pathology.uc.edu/divisions/comppath.asp


    Transgenic Core Facility at CCHMC Research Foundation

    1. Purpose:  Generation of genetically altered mice and rabbits; embryo cryopreservation

    2. Location:  Department of Pediatrics
      CHMC/CHRF, TCHRF
      Room 2047 CHRF
      ML# 7007

    3. Director:  Dr. Richard A. Lang
      Phone: 513-636-7030

      Operations
      Sandy Falcone
      Email: alexandra.falcone@cchmc.org
      Phone: 513-636-7098

    4. Frequency of use:  Currently CHRF 80%; UC 15%; other 5%

    5. Accessible:  Accessible to faculty from outside CHRF



    Transgenic Mouse Core

    1. Purpose:  Production of transgenic mice bearing exogenous genes

    2. Location:  Department of Molecular Genetics, Biochemistry and Microbiology
      Room 2002 MSB
      ML# 0524

    3. Director:  Dr. Jerry Lingrel
      Phone: 513-558-5324

      Director
      Jon Neumann
      Email: neumanjc@email.uc.edu
      Phone: 513-558-5294

    4. Fee structure:  Supported by funds from the POE, MMD and user fees

    5. Frequency of use:  Used full-time; roughly 50-50 division between internal and external

    6. Accessible:  Accessible to all funded investigators

    7. Website:  http://www.med.uc.edu/htdocs/medicine/trans_web/home.html


    Transgenic Mouse Physiology Core

    1. Purpose:  Analysis of a variety of physiological (cardiac, renal, pulmonary) functions in intact transgenic mice and development of new technologies/instrumentation related thereto.

    2. Location:  Department of Molecular and Cellular Physiology
      MSB 4056
      ML# 0576

    3. Director:  Dr. John Lorenz
      Email: john.lorenz@uc.edu
      Phone: 513-558-3097

    4. Fee structure:  Either joint grants or user fees; presently derives some fixed-term support from the Dean’s office

    5. Frequency of use:  20% Physiology Department, 20% CHRF and 60% other departments

    6. Accessible:  Accessible to all



Viral Vector

    Comparative Pathology

    1. Purpose:  Consult and carry out pathology studies on animals or animal tissues.

    2. Location:  Medical Science Building 1054 and 1109.

    3. Director:  Dr. Greg Boivin, 558-9156; boivingp@ucmail.uc.edu
      Laboratory Supervisor: Rita Angel and Yvette Doty, 558-4436

    4. Fee structure:  Fee for service based on established schedule. Contact Dr. Boivin for more information. Technical and Professional support can also be added to grants directly.

    5. Frequency of use:  1500 cases are received annually: 60% Medical Center, 25% Children’s Hospital, 10% VA Medical Center, 5% Shriners, West Campus and other Universities.

    6. Accessible:  Accessible and available for sample submission during regular business hours.

    7. Other:  Types of Preparations: Animal necropsy, tissue processing, embedding, sectioning, staining, immunohistochemistry, frozen-sectioning

      Some studies available: Animal necropsy, tissue processing, embedding, sectioning, staining, immunohistochemistry, frozen-sectioning, photographs.

    8. Website:  http://pathology.uc.edu/divisions/comppath.asp


    Viral Vector Core

    1. Purpose:  The Viral Vector Core (VVC) is a joint effort of the Divisions of Experimental Hematology and Pulmonary Biology and provides research-grade Retroviral and Adenoviral Vectors based on a fee-for-service model to support and enhance research at the Cincinnati Children's Hospital, the University of Cincinnati Medical Centers, the Genome Research Institute and the Cincinnati Children's Hospital Research Foundation. Services can be requested by faculty or staff members.

    2. Location:  Research-grade Retroviral and Adenoviral vectors are produced in physically separated, dedicated Biosafety Level 2 (BL2) laboratories. Retroviral Vectors are produced in the Vector Production Facility (VPF), located on the third floor of the Hoxworth Building or in the Cincinnati Children's Hospital Research Building under the direction of Han van der Loo, PhD. Adenoviral vectors are prepared in a BL2 laboratory located on the 4th floor of the Research Building directed by Bruce Trapnell, MD.

    3. Equipment:  The VVC began operations in the fall of 2003 and has since produced 120 different viral vector products. We are offering the following research level services: 1. Production of retroviral and adenoviral vectors using vector plasmids provided by the investigator. 2. Generation of stable retroviral producer cell lines. 3. Vector titration. 4. Mycoplasma and sterility testing. 5. Plasmid purification. 6. Vector concentration (select envelopes). 7. Lentiviral vector generation and RCL testing. (to be available in Fall of 2004.)

    4. Director:  The Viral Vector Core and Retroviral Vector component of the Core are under the direction of Dr. Han van der Loo, Division of Experimental Hematology, Department of Pediatrics ( Han.vanderloo@cchmc.org ). Ph 513-475-4093 The adenoviral vector component of the core is managed by Dr. Bruce Trapnell, Division of Pulmonary Biology, Department of Pediatrics ( Bruce.Trapnell@cchmc.org ). Ph 513-636-6361

    5. Fee structure:  The costs for services are charged to the Division's or Department's budget through the CCHMC Research Administration Service Center. Overall, fees have been subsidized to allow cost efficiency to investigators. Contact the VVC for a pricing overview or for a formal Quote.

    6. Accessible:  Investigators requiring Vector Core services should complete and submit a Request Form for Retroviral or Adenoviral Vector. Request forms can be downloaded from the Vector Core website at http://www.cincinnatichildrens.org/research/div/exp-hematology/translational/vpf/vvc/default.htm For general information regarding the Viral Vector Core, or for further information regarding the Retroviral Vector component of the Core, please contact Dr. Han van der Loo at 513-475-4093. For further information regarding the Adenoviral Vector component of the Core, please contact Dr. Bruce Trapnell at 513-636-6361.

    7. Website:  http://www.cincinnatichildrens.org/research/div/exp-hematology/translational/vpf/vvc/default.htm