Live Microscopy Core

1. Purpose:  The facility has two advanced light microscopes optimized for work with living cells and tissues.  All data is automatically backed up after every user session to a remote computer that allows users password-controlled access to archived data for a month.
   
   
2. Location:  Department of Molecular & Cellular Physiology
   MSB 4105
   ML #0576
   
   
3. Equipment: 
   1. Zeiss LSM510 META confocal microscope
      1. Available all mornings and weekends by arrangement
      2. Microscope:  Zeiss Axiovert 200 inverted microscope platform
      3. Microscope objectives: 40X C-Apochromat (water immersion), 10X C-Apochromat (water immersion)
      4. Two conventional confocal detectors
      5. META detector that allows spectral analysis of emission over the range of 460-800 nm with roughly 10 nm resolution
      6. Transmitted light detector for laser-light Nomarski (differential interference contrast) imaging
      7. Fluorescence filter blocks for direct visualization of double and triple labeled samples by eye
   2. Zeiss LSM510 NLO two-photon and confocal microscope
      1. Available 24/7
      2. Microscope: Zeiss Axiovert 200 inverted microscope platform
      3. Dark Environmental chamber: encloses microscope to allow 37°C control and keep stray light out of detecton
      4. Microscope objectives: 40X C-Apochromat (water immersion), 10X C-Apochromat (water immersion)
      5. Lasers for excitation of fluorescence: Ar (458, 477, 488, and 514 nm), green HeNe (543nm) and red HeNe (633 nm), Ti-Sa (700-990 nm).
      6. Two non-descanned detectors that allow collection of light from deep tissue two-photon imaging with higher efficiency than confocal detectors.
      7. Transmitted light detector for laser-light Nomarski (differential interference contrast) imaging
      8. Fluorescence filter blocks for direct visualization of double and triple labeled samples by eye
   
   
   
4. Director:  Manager
   Chet Closson
   Email: clossoct@uc.edu
   Phone:  513-558-3791
   
   Director
   Marshall Montrose, Ph.D.
   Email: mhm@uc.edu
   Phone:  513-558-5636
   
   
5. Fee structure:  $250/person user training for novices. $100/person two-photon training and “entrance exam” for users familiar with Zeiss LSM. $30/hour usage fee on either microscope M-F 8-5. $20/hour usage fee on either microscope off-hours and weekends. CD-R or CD-RW provided at cost to users to facilitate data storage.
   
   
6. Accessible:  The facility is self-serve, open only to users who have undergone full training so that they can work independently. Card key access provides 24/7 access to the facility for trained users.
   
   
7. Website:  http://www.med.uc.edu/physiology/RESOURCES_microscopy.htm