Proteomics

1. Purpose: 

   Our mission is to provide expertise & coordinate services in proteomics and biological mass spectrometry to the UC & CCHMC research community.  This includes both fee-for-service projects and collaborative research projects.

   
   
   
2. Location: 

   UC Reading Campus
   MDI, Building B, Rooms 126-131
   Lab Phone:  513-558-4057
   Director Phone: 513-558-7102
   ML 0505 

   
   
   
3. Equipment: 

   
   Mass Spectrometers
   (1)     AB 4800 MALDI-TOF/TOF MS w/Tempo nanoLC
   (2)     Thermo LTQ linear Ion-Trap-MS w/Eksigent nanoLC
   (3)     ThermoLCQDeca XP Ion-Trap-MS w/Dionex nanoLC
   (4)     MicroMass/Waters LCT (LC-ESI-TOF-MS) 

    

   2-D gel equipment
   (1)     Four Amersham Multiphor II isoelectric focusing systems
   (2)     Four Genomic Solutions Investigator 2-D system
   (3)     Compugen Z3 Gel Analysis Imaging System
   (4)    GE Healthcare (Amersham) DIGE 2-D gel analysis system
   (5)     Fuji Fluorescence scanner

   
   
   
4. Director: 

   Director: 
   Kenneth D. Greis, Ph.D
   Associate Professor of Cancer and Cell Biology
   University of Cincinnati College of Medicine
   2180 E. Galbraith Road, Rm B109
   Cincinnati,OH 45237
   513 558-5061 Fax
   513 558-7102
   email: ken.greis@uc.edu
   
   Lab Personnel
   Wendy Haffey, Ph.D.
   Mass Spectrometry
   Phone: 513-558-4057
   
   Hongjun Shu, Ph.D.
   Mass Spectrometry
   Phone: 513-558-4057
   
   Mike Wyder, M.S.
   2-D gel separation, Image analysis, Mass Spectrometry
   Phone: 513-558-4057
   
   Kelly Rask, M.S.
   Mass Spectrometry, 2-D gel separation, Image analysis
   Phone: 513-558-4057

   
   
   
5. Fee structure: 

   Proteomics Services	Internal cost per item
   1.   In gel digestion and recovery	$25
   2.   MALDI-TOF/TOF protein ID	$50
   3.   LC-MS/MS Protein ID	$125
   4.   LC-MALDI-TOF/TOF	130$
   5.   MALDI-TOF of intact protein	$50
   6.   LC-ESI-TOF of proteins	$70
   7.   Serum Immunodepletion	$70
   8.   Protein Concentration Assay	$15
   9.   DIGE labeling per Rxn pair	$290
   10. Large 2D gel separation	$130
   11. Mini 2D gel separation	$70
   12. Gel Stain (Silver/Coomassie)	$25
   13. Gel stain (Sypro/ProQ Diamond)	$90
   14. Fluorescent Image Capture	$35
   15. Image analysis per hour	$30
   16. Others Services
   (Time and material costs)	Contact the Director
   All rates are effective 9/1/2009 and subject to change

   
   
   
6. Accessible: 

   Monday through Friday, 7:00 AM to 6:00 PM. Call the Director to schedule an appointment for a consultation or for a quotation for on services. 

   
   
   
7. Website:  http://cancerbiology.uc.edu/about-cancer--cell-biology/core-facilities/proteomics.aspx