Proteomics

1. Purpose: 
   Our mission is provide expertise & coordinate services in proteomics and biological mass spectrometry to the UC & CCHMC research community.  This includes both fee-for-service projects and collaborative research projects.
   
   
2. Location: 
   Proteomics Laboratory
   GRI, Building B, Rooms 126-131
   Lab Phone:  513-558-4057
   Director Phone: 513-558-7102
   ML 0505
   
   
3. Equipment: 
   Mass Spectrometers
   (1)     AB 4800 MALDI-TOF/TOF MS w/Tempo nanoLC
   (2)     Thermo LTQ linear Ion-Trap-MS w/Eksigent nanoLC
   (3)     ThermoLCQDeca XP Ion-Trap-MS w/Dionex nanoLC
   (4)     MicroMass/Waters LCT (LC-ESI-TOF-MS) 
   

   2-D gel equipment
   (1)     Four Amersham Multiphor II isoelectric focusing systems
   (2)     Four Genomic Solutions Investigator 2-D system
   (3)     Compugen Z3 Gel Analysis Imaging System
   (4)    GE Healthcare (Amersham) DIGE 2-D gel analysis system
   (5)     Fuji Fluorescence scanner
   
   
   

4. Director: 
   Kenneth D. Greis, Ph.D
   Associate Professor
   Department of Genome Science
   Genome Research Institute
   2180 E. Galbraith Road, Rm B109
   Cincinnati,OH 45237
   513 558-5061 Fax
   513 558-7102
   email: ken.greis@uc.edu
   
   Lab Personnel
   Wendy Dominick, Ph.D.
   Mass Spectrometry
   Phone: 513-558-4057
   
   Hongjun Shu, Ph.D.
   Mass Spectrometry
   Phone: 513-558-4057
   
   Mike Wyder, M.S
   2-D gel separation, Image analysis, Mass Spectrometry
   Phone: 513-558-4057
   
   Kelly Rask, M.S
   Mass Spectrometry, 2-D gel separation, Image analysis
   Phone: 513-558-4057
   
   
5. Fee structure: 

   Proteomics Services	cost per item
   1.  In gel digestion and recovery	$20
   2.  MALDI-TOF/TOF protein ID	$49
   3.  LC-MS/MS Protein ID	$131
   4.  LC-MALDI-TOF/TOF	$163
   5.  MALDI-TOF of intact protein	$54
   6.  LC-ESI-TOF of proteins	$64
   7.  Serum Immunodepletion	$70
   8.  Protein Concentration Assay	$9
   9.  DIGE labeling per Rxn pair	$260
   10. 2D gel separation	$111
   11. Gel Stain (Silver/Coomassie)	$17
   12. Gel stain (Sypro/ProQ Diamond)	$76
   13. Fluorescent Image Capture	$41
   14. Image analysis per hour	$30
   15. Others Services
   (Time and material costs)	Contact the Director

   
   
   
6. Frequency of use:  50% UC, 50% CHMC
   
   
7. Accessible:  Monday through Friday, 7:00 AM to 6:00 PM. Call the Director to schedule an appointment for a consultation or for a quotation for on services.
   
   
8. Website:  http://www.gri.uc.edu/extended_info.php?c2h_id=3&extended=Proteomics